Journal: HardwareX

Article Title: The FAST Pump, a low-cost, easy to fabricate, SLA-3D-printed peristaltic pump for multi-channel systems in any lab

doi: 10.1016/j.ohx.2020.e00115

Figure Lengend Snippet: Results of the Single enzyme-linked immunosorbent assay for the detection of Tau protein. A)-D) show representative fluorescence micrographs of a microdroplet array (MDA) functionalized for the immobilization and quantification of Tau protein. A) 100 fM. B) 1 fM. C) 10 aM. D) Negative control. The MDA is imaged on a Zeiss Axio ObserverZ1 microscope equipped with a Zeiss Axiocam MRm B/W camera using 20x magnification. Excitation wavelength is 555 nm and emission wavelength is 583 nm. The number of fluorescent droplets, taken as a measurement of the concentration of the target analyte in the sample, is counted using pattern recognition in the software ImageJ after binarizing the signal from the spots with an intensity threshold applied to all the fluorescence micrographs. E) Standard curve from quantification of the number of spots in the MDA for each concentration (n = 2).

Article Snippet: The MDA is imaged on a Zeiss Axio ObserverZ1 microscope equipped with a Zeiss Axiocam MRm B/W camera using 20x magnification.

Techniques: Enzyme-linked Immunosorbent Assay, Fluorescence, Negative Control, Microscopy, Concentration Assay, Software